Human HA1.7 TCR beta chain expressed on transgenic mouse cells.
Human T cell receptor (TCR) is a T cell surface expressed heterodimer of a/b or g/d chains. TCRs recognize antigen peptides bound to MHC molecules forming the basis of antigen specific response by T cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml, 80ul labels 5x10e5 cultured PBMC cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 ficoll prepared PBMC cells were washed and incubated 45 minutes on ice with 80ul of T2040-04 at 5ug/ml. 2. Cells were washed twice and incubated secondary reagent, after which they were washed three times, fixed and analyzed by FACS using a lymphoid gate. 3 A net 43% sub population of the cells stained positive with a mean shift of 1.541 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[7H199]
Isotype:
IgG2a
Purity:
Purified by Protein A affinity chromatography from tissue culture supernatant.
Form:
Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.
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