Human HA1.7 TCR beta chain expressed on transgenic mouse cells
Human T cell receptor (TCR) is a T cell surface expressed heterodimer of a/b or g/d chains. TCRs recognize antigen peptides bound to MHC molecules forming the basis of antigen specific response by T cells. Antibody is mitogenic for T cells expressing TCR C beta 1. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ug/ml Optimal dilutions to be determined by the researcher. Protocol: 1. Five x 10e5 ficoll prepared human PBMC were washed and pre incubated 10 minutes with 20ul of 300ug/ml human IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80ul T2040-04B at 5ug/ml. 2. Cells were washed twice and incubated with 50ul of SA(PE) after which they were washed three times, fixed and analyzed by FACS. 3. A 34% sub population of the cells stained positive with a mean shift of 2.09 log10 fluorescent units when compared to a Mouse IgG2a/Biotin negative control. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml T2040-04 antibody. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[7H199]
Isotype:
IgG2a
Purity:
Purified by Protein A affinity chromatography from tissue culture supernant.
Form:
Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride 5% glycerol, 0.2% BSA, 0.04% sodium azide, low FBS. Labeled with Biotin.
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