TdT is a DNA polymerase responsible for the catalysis of non-reversible addition of deoxynucleotides to the 3 end hydroxy groups of DNA. TdT levels are enhanced in all forms of acute lymphoblastic leukemia (ALL) and in a significant number of chronic lymphoblastic leukemia cases (CML). TdT is not expressed in the majority of myeloid leukemias, non-Hodgkins lymphomas or mature lymphoid leukemias. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat, 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
