Recombinant corresponding to aa28-298 from mouse Testisin expressed in NSO cell line.
Testisin is a serine protease encoded by the PRSS21 gene. Testisin is similar in structure to tryptases, and is also known as Tryptase-4. It is predicted to have substrate specificity similar to that of trypsin. Testisin is most highly expressed in testicular germ cells, but is also present in eosinophils. Testisin is known to be required for spermatogenesis, and deficiencies of the enzyme can result in infertility. Testisin is anchored to the cell surface through a C-terminal glycosylphosphatidylinositol anchor. Recombinant mouse Testisin is expressed with a truncated C-terminus, resulting in its secretion. The recombinant protein was purified as the zymogen and can undergo autoactivation under the appropriate conditions. Over aa28-298, mouse Testisin shares 92%aa identity with rat Testisin. Applications: Suitable for use in Immunohistochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5mg/ml Immunohistochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
