Synthetic peptide corresponding to aa31-49, AQKLGVEQPTLYWHVKNKR of the 22kD Tet repressor protein.
The Tet repressor protein (TetR) regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Detection limit - 0.8ng ELISA: Detection limit - 0.2ng Optimal dilution to be determined by researcher Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
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