Tetrahydrocannabinol, D9 (THC) (Not for Export EU), Sheep
Biozol Catalog Number:
USB-T2970-07
Supplier Catalog Number:
T2970-07
Alternative Catalog Number:
USB-T2970-07-100
Manufacturer:
US Biological
Host:
Sheep
Category:
Antikörper
Application:
ELISA
Immunogen:
D9-Tetrahydrocannabinol
Cannabinoids, a group of C21 compounds present in Cannabis sativa L., their carboxylic acids, analogs, and transformation products, are the active ingredients found in hasish and marihuana. (-)-trans-D9-tetrahydrocannabinol (D9-THC) is the major psychopharmacologically active component of cannabis. Cannabis affect cognition and memory, euphoria and sedation, and antinociception (analgesia) without the respiratory depression problems associated with opioid analgesics. D9-THC is also immunosuppressive and impairs cell-mediated immunity, humoral immunity and cellular defenses against a variety of infectious agents both in vivo and in vitro. To date, two sub-types of the G-protein coupled cannabinoid receptor, CB1 and CB2, have been identified. The first brain-derived endogenous cannabinoids, an unsaturated fatty-acid ethanolamide, arachidonylethanolamide (AEA, also called anandamide) was found in brain. AEA has higher affinity for the CB1 than for the CB2. THC is rapidly adsorbed by the lungs and almost completely metabolized. The major metabolite is 11-nor-delta-9-tetrahydrocannbinol-9-carboxylic acid and is found with other metabolites in plasma, feces, and urine. THC-metabolites can be detected within hours or exposure and may be detectable for several days. A cut-off level of 50ng/ml marijuana metabolites has been selected as the initial screening level by the US Dept. of Health. Antibodies to THC were produced in sheep (polyclonal) or in mouse (monoclonal). Mouse Monoclonal (IgG1) have been purified using Protein A/G affinity chromatography. Applications: Suitable for use in ELISA and Western Blot Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 ELISA: 1:10,000-1:100,000 using 50-100ng of THC-BSA or other protein conjuagtes/well Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified using Protein A/G affinity chromatography
Form:
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
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