Recombinant protein corresponding to Lys2-Cys689 from human THOP-1, expressed in E. coli.
Thimet oligopeptidase (THOP1), also known as endopeptidase EC 3.4.24.15 (EP24.15), is a zinc peptidase of the M3 family that also includes neurolysin/EC 3.4.24.16 and mitochondrial intermediate peptidase. Widely expressed by mammalian tissues and reported to be present in different subcellular locations, THOP1 is primarily a cytoplasmic enzyme capable of hydrolyzing a number of bioactive peptides and peptides released from by the proteosome, limiting antigenic presentation by MHC class I molecules. The amino acid sequence of human THOP1 is 90% and 86% identical to that of dog/mouse and rat. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml tested with recombinant human Thimet Oligopeptidase/THOP1. Immunoprecipitation: 25ug/ml in conditioned cell culture medium spiked with recombinant human Thimet Oligopeptidase/THOP1. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
