Recombinant protein corresponding to Leu23-Lys211 from mouse TFPI-2 expressed in NS0 cells.
TFPI-2 (tissue factor pathway inhibitor-2, also placental protein 5/PP5) is a secreted, presumably 27-34kD glycoprotein member of the Kunitz domain-containing proteinase inhibitor family. In mouse, it is principally produced by endothelium, hepatocytes and synctiotrophoblasts, and serves as an inhibitor of multiple serine proteinases. Mature mouse TFPI-2 is 208aa in length. It contains three Kunitz inhibitor domains, the most N-terminal of which (aa36-86) is crucial to inhibitor activity. Over aa23-211, mouse TFPI-2 shares 86% and 58% aa sequence identity with rat and human TFPI-2, respectively. Applications: Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml. Detection limit for rmTFPI-2 is ~20ng/lane and 2ng/lane under non-reducing and reducing conditions. ELISA (direct): 0.5-1ug/ml. Detection limit for rmTFPI-2 is ~0.1ng/well. Immunoprecipitation: 25ug/ml Immunoprecipitates rmTFPI-2 from conditioned media of transfected NS0 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
