TIMP-1and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry: Frozen sections or acetone-fixed tissues. Formalin-fixed, paraffin-embedded sections are usually not reactive. PLP fixation is suggested as an alternative for paraffin-embedded sectons. Western Blot: 1:1,000. Identifies a band of approximately 30KDa. Note TIMP-1 is found in very low concentrations in most cells and tissues. Positive control is HT1080 cells treated with PMA or recombinant TIMP-1 protein. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
