Toll Like Receptor 3 (TLR3), IgG1, Clone: [TLR3.7], Mouse, Monoclonal
Biozol Catalog Number:
USB-T8050-24
Supplier Catalog Number:
T8050-24
Alternative Catalog Number:
USB-T8050-24-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
FC, IHC, WB
Immunogen:
Ba/F3 cells that stably expressed human Flag-tagged TLR3
Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. As investigated so far all functional characterized TLR signal via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. Viral replication within infected cells results in generation of dsRNA which can stimulate immune cells. Recent data show that TLR3 (CD283) acts as a receptor involved in the recognition of dsRNA, indicating a role for TLR3 in viral recognition and innate defense. Applications: Can be used for intracellular Flow Cytometry, Immunohistochemistry and Western Blot. Furthermore the antibody is useful for in vitro inhibition by the ligand poly(I):poly(C). Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10 Western Blot: 1:10 Immunohistochemistry: paraffin and frozen For inhibition studies dilutions have to be made according to the amounts ligand to be inactivated. Optimal dilutions to be determined by the researcher. Positive Control: Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells Negative Control: HEK293 cells. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[TLR3.7]
Isotype:
IgG1
Purity:
Purified by Protein G affinity chromatography.
Form:
Supplied as a liquid in PBS, 0.1% BSA.
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