TLR4 (Toll Like Receptor 4) (MaxLight 550), Rabbit
Biozol Catalog Number:
USB-T8050-34E-ML550
Supplier Catalog Number:
T8050-34E-ML550
Alternative Catalog Number:
USB-T8050-34E-ML550-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
FLISA, WB
Immunogen:
Synthetic peptide derived from the carboxyl termini of various human TLR4 (Toll-like receptor 4) isoforms A-D.
The Drosophila receptor, Toll, was initially found to be essential for protective immunity to fungal infections in flies. Toll-like homologs have been identified in organisms as disparate as plants, insects and mammals. Of the ten known members of the mammalian TLR family, four have been shown to initiate inflammatory responses upon recognition of pathogen derived substances. Toll-like receptors (TLRs) are family of signaling molecules that are required for innate responses to pathogen-derived substances such as lipopolysaccharides (LPS), proteoglycans and bacterial DNA. Innate immunity plays an essential role in the host defense and is crucial for the generation of primary adaptive responses. TLR expression is highly regulated in monocytes and dendritic cells during differentiation and maturation. Mammalian cells respond to LPS by activating Toll-like receptor 4 (TLR4). TLR4 was first described as a member of a family of type I integral membrane proteins that contain numerous leucine-rich motifs on their extracellular portions and an intracellular signaling domain homologous to that of the IL-1 receptor. TLR4 initiates a cascade of serine/threonine kinases such as IL-1 receptor-associated kinase (IRAK) in response to LPS, resulting in downstream activation of NF-kB and c-Jun NH2-terminal kinase which eventually leads to the transcription of genes involved in inflammation. TLR4 requires an accessory protein, MD-2 to respond efficiently to LPS. MD-2 binds to the extracellular domains of both TLR2 and TLR4, causing their surface expression levels to increase. Secreted MD-2 is a large polymeric protein that confers LPS sensitivity to TLR4. LPS binds directly to the TLR4/MD-2 complex and both molecules are in close proximity to the bound LPS. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Applications: Suitable for use in FLISA and Western Blot. Other applications have not been tested. Recommended Dilution: FLISA: 0.1-1ug/ml Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Purity:
Purified by epitope affinity chromatography
Form:
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight(TM)550.
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