Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR4 (CD284) has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of gram-negative bacteria. Applications: Suitable for use in Flow Cytometry, Functional Studies, ELISA, Immunofluorescence, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 300,000 cells/50ul were stained with 2ug for 30 minutes at 4C Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[6A639 HTA125]
Isotype:
IgG2a
Purity:
Purified by Protein G affinity chromatography.
Form:
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
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