Tumor Necrosis Factor Receptor 2 Soluble, Recombinant, Human (sTNFR 2)
Biozol Catalog Number:
USB-T9162-75
Supplier Catalog Number:
T9162-75
Alternative Catalog Number:
USB-T9162-75-20, USB-T9162-75-100
Manufacturer:
US Biological
Category:
Molekularbiologie
TNFRII is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF-alpha but manifests a lower affinity to TNF-beta. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. Soluble TNFRII is capable of inhibiting TNF-alpha induced activities by acting as a decoy receptor. Recombinant human sTNFRII is an 18.9kD protein (174 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFRII protein. Recombinant protein corresponding to 174aa from the cysteine rich ligand binding portion of the extracellular domain of the human TNFRII protein expressed in E. coli Biological Activity: Determined by its inhibitory effect on the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25ng/ml of recombinant human TNF-alpha is 0.125ug/ml. Amino Acid Sequence: MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug)
Form:
Suppllied as a lyophilized powder from 10mM sodium phosphate, pH 8.0, 50mM sodium chloride. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex.
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