Hemoglobin subunit alpha (HBA1) Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer:
ABB-A14551
- Bilder (1)
| Artikelname: | Hemoglobin subunit alpha (HBA1) Rabbit pAb, Unconjugated, Polyclonal |
| Artikelnummer: | ABB-A14551 |
| Hersteller Artikelnummer: | A14551 |
| Alternativnummer: | ABB-A14551-20UL,ABB-A14551-100UL,ABB-A14551-1000UL,ABB-A14551-500UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | HBH, ECYT7, HBA-T3, METHBA, Hemoglobin subunit alpha (HBA1) |
| The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5 untranslated regions and the introns, but they differ significantly over the 3 untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin, alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1, some nondeletion alpha thalassemias have also been reported. |
| Application Verdünnung: | WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human. Shipping: Ice Bag |
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Western blot analysis of lysates from K-562 cells, using Hemoglobin subunit alpha (HBA1) Rabbit pAb (A14551) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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