Enables several functions, including GTP-dependent protein binding activity, guanyl-nucleotide exchange factor activity, and phosphatidic acid binding activity. Involved in microvillus assembly, positive regulation of GTPase activity, and protein localization to plasma membrane. Located in several cellular components, including apical plasma membrane, centrosome, and endocytic vesicle.
WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:
Cross-Reactivity: Mouse,Rat. ResearchArea: Signal Transduction,G protein signaling,Small G proteins. Shipping: Ice Bag
Western blot analysis of various lysates using RAPGEF6 Rabbit pAb (A17161) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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