Paxillin Rabbit pAb, Unconjugated, Polyclonal

Artikelnummer: ABB-A18181
Artikelname: Paxillin Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer: ABB-A18181
Hersteller Artikelnummer: A18181
Alternativnummer: ABB-A18181-20UL,ABB-A18181-100UL,ABB-A18181-1000UL,ABB-A18181-500UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: Paxillin, PXN, paxillin
This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445).
Klonalität: Polyclonal
Molekulargewicht: 65kDa
NCBI: 5829
UniProt: P49023
Reinheit: Affinity purification
Sequenz: MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNP
Target-Kategorie: PXN
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse. ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,PI3K-Akt Signaling Pathway,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Cytoskeleton,Actins,Extracellular Matrix. Shipping: Ice Bag
Western blot analysis of lysates from PC-3 cells, using Paxillin Rabbit pAb (A18181) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.