Paxillin Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A18181
Article Name: Paxillin Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A18181
Supplier Catalog Number: A18181
Alternative Catalog Number: ABB-A18181-20UL,ABB-A18181-100UL,ABB-A18181-1000UL,ABB-A18181-500UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: Paxillin, PXN, paxillin
This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445).
Clonality: Polyclonal
Molecular Weight: 65kDa
NCBI: 5829
UniProt: P49023
Purity: Affinity purification
Sequence: MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNP
Target: PXN
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse. ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,PI3K-Akt Signaling Pathway,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Cytoskeleton,Actins,Extracellular Matrix. Shipping: Ice Bag
Western blot analysis of lysates from PC-3 cells, using Paxillin Rabbit pAb (A18181) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.