ATG12 Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A19610
Artikelname: ATG12 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A19610
Hersteller Artikelnummer: A19610
Alternativnummer: ABB-A19610-100UL,ABB-A19610-20UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: APG12, FBR93, APG12L, HAPG12, ATG12
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.
Klonalität: Monoclonal
Molekulargewicht: 15 kDa
NCBI: 9140
UniProt: O94817
Reinheit: Affinity purification
Target-Kategorie: ATG12
Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse. ResearchArea: Cancer,Tumor suppressors,Signal transduction,Cell biology developmental biology,Apoptosis,Autophagy,Ubiquitin,Endocrine metabolism,Mitochondrial metabolism,Immunology inflammation,Cardiovascular,Heart. Shipping: Ice Bag
Western blot analysis of various lysates using ATG12 Rabbit mAb (A19610) at 1:1000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60 S.