ATG12 Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A19610
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| Article Name: | ATG12 Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A19610 |
| Supplier Catalog Number: | A19610 |
| Alternative Catalog Number: | ABB-A19610-100UL,ABB-A19610-20UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | APG12, FBR93, APG12L, HAPG12, ATG12 |
| Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy. |
| Application Dilute: | WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse. ResearchArea: Cancer,Tumor suppressors,Signal transduction,Cell biology developmental biology,Apoptosis,Autophagy,Ubiquitin,Endocrine metabolism,Mitochondrial metabolism,Immunology inflammation,Cardiovascular,Heart. Shipping: Ice Bag |
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Western blot analysis of various lysates using ATG12 Rabbit mAb (A19610) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60 S. |
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