Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation:
Unconjugated
Biotin is an organic heterobicyclic compound that consists of 2-oxohexahydro-1H-thieno[3,4-d]imidazole having a valeric acid substituent attached to the tetrahydrothiophene ring. The parent of the class of biotins. It has a role as a prosthetic group, a coenzyme, a nutraceutical, a human metabolite, a Saccharomyces cerevisiae metabolite, an Escherichia coli metabolite, a mouse metabolite, a cofactor and a fundamental metabolite. It is a member of biotins and a vitamin B7. It is a conjugate acid of a biotinate.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[ARC50695]
Isotyp:
IgG1
Reinheit:
Affinity purification
Target-Kategorie:
Biotin
Antibody Type:
Primary Antibody
Application Verdünnung:
WB,1:1000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:
Cross-Reactivity: Other (Wide Range Predicted). ResearchArea: Tags and Cell Markers, Epitope Tags, Conjugates, Signal Transduction, Metabolism Vitamins / Minerals, Metabolism Pathways and Processes Cofactors, Vitamins / minerals Vitamins / minerals. Shipping: Ice Bag
Western blot analysis of various lysates, using Biotin Rabbit mAb (A20684) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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