Biotin Rabbit mAb, Unconjugated

Catalog Number: ABB-A20684
Article Name: Biotin Rabbit mAb, Unconjugated
Biozol Catalog Number: ABB-A20684
Supplier Catalog Number: A20684
Alternative Catalog Number: ABB-A20684-100UL,ABB-A20684-20UL,ABB-A20684-1000UL,ABB-A20684-500UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Biotin is an organic heterobicyclic compound that consists of 2-oxohexahydro-1H-thieno[3,4-d]imidazole having a valeric acid substituent attached to the tetrahydrothiophene ring. The parent of the class of biotins. It has a role as a prosthetic group, a coenzyme, a nutraceutical, a human metabolite, a Saccharomyces cerevisiae metabolite, an Escherichia coli metabolite, a mouse metabolite, a cofactor and a fundamental metabolite. It is a member of biotins and a vitamin B7. It is a conjugate acid of a biotinate.
Clonality: Monoclonal
Clone Designation: [ARC50695]
Isotype: IgG1
Purity: Affinity purification
Target: Biotin
Antibody Type: Primary Antibody
Application Dilute: WB,1:1000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Other (Wide Range Predicted). ResearchArea: Tags and Cell Markers, Epitope Tags, Conjugates, Signal Transduction, Metabolism Vitamins / Minerals, Metabolism Pathways and Processes Cofactors, Vitamins / minerals Vitamins / minerals. Shipping: Ice Bag
Western blot analysis of various lysates, using Biotin Rabbit mAb (A20684) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.