BCKDK Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A20909
Artikelname: BCKDK Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A20909
Hersteller Artikelnummer: A20909
Alternativnummer: ABB-A20909-100UL,ABB-A20909-20UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: BDK, BCKDKD, BCKDK
The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene.
Klonalität: Monoclonal
Molekulargewicht: 46kDa
NCBI: 10295
UniProt: O14874
Reinheit: Affinity purification
Target-Kategorie: BCKDK
Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Endocrine Metabolism,Mitochondrial metabolism,Mitochondrial markers,Amino acid metabolism. Shipping: Ice Bag
Western blot analysis of various lysates using BCKDK Rabbit mAb (A20909) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.