BCKDK Rabbit mAb, Unconjugated, Monoclonal

Catalog Number: ABB-A20909
Article Name: BCKDK Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number: ABB-A20909
Supplier Catalog Number: A20909
Alternative Catalog Number: ABB-A20909-100UL,ABB-A20909-20UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: BDK, BCKDKD, BCKDK
The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene.
Clonality: Monoclonal
Molecular Weight: 46kDa
NCBI: 10295
UniProt: O14874
Purity: Affinity purification
Target: BCKDK
Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Endocrine Metabolism,Mitochondrial metabolism,Mitochondrial markers,Amino acid metabolism. Shipping: Ice Bag
Western blot analysis of various lysates using BCKDK Rabbit mAb (A20909) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.