HIST1H3D Rabbit pAb, Polyclonal

Artikelnummer: ABB-A21306
Artikelname: HIST1H3D Rabbit pAb, Polyclonal
Artikelnummer: ABB-A21306
Hersteller Artikelnummer: A21306
Alternativnummer: ABB-A21306-100UL,ABB-A21306-20UL,ABB-A21306-1000UL,ABB-A21306-500UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Alternative Synonym: H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A, HIST1H3D
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Klonalität: Polyclonal
Molekulargewicht: 15KDa
NCBI: 8290
UniProt: Q16695
Reinheit: Affinity purification
Sequenz: MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA
Target-Kategorie: HIST1H3D
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat,Other (Wide Range Predicted). Shipping: Ice Bag
Western blot analysis of lysates from C6 cells, using HIST1H3D Rabbit pAb (A21306) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.