HIST1H3D Rabbit pAb, Polyclonal
Catalog Number:
ABB-A21306
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| Article Name: | HIST1H3D Rabbit pAb, Polyclonal |
| Biozol Catalog Number: | ABB-A21306 |
| Supplier Catalog Number: | A21306 |
| Alternative Catalog Number: | ABB-A21306-100UL,ABB-A21306-20UL,ABB-A21306-1000UL,ABB-A21306-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Alternative Names: | H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A, HIST1H3D |
| Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
| Application Dilute: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat,Other (Wide Range Predicted). Shipping: Ice Bag |
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Western blot analysis of lysates from C6 cells, using HIST1H3D Rabbit pAb (A21306) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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