NANOG Rabbit pAb, Unconjugated, Polyclonal

Artikelnummer: ABB-A22115
Artikelname: NANOG Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer: ABB-A22115
Hersteller Artikelnummer: A22115
Alternativnummer: ABB-A22115-100UL,ABB-A22115-20UL,ABB-A22115-500UL,ABB-A22115-1000UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Mouse
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: ENK, Stm1, ecat4, 2410002E02Rik, NANOG
The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Several transcript variants encoding different isoforms have been found for this gene.
Klonalität: Polyclonal
Molekulargewicht: 34kDa
NCBI: 71950
UniProt: Q80Z64
Reinheit: Affinity purification
Sequenz: LKTSNGLIQKGSAPVEYPSIHCSYPQGYLVNASGSLSMWGSQTWTNPTWSSQTWTNPTWNNQTWTNPTWSSQAWTAQSWNGQPWNAAPLHNFGEDFLQPYVQLQQNFSASDLEVNLEATRESHAHFSTPQALELFLNYSVTPPGEI
Target-Kategorie: Nanog
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human. Shipping: Ice Bag
Western blot analysis of various lysates, using NANOG Rabbit pAb (A22115) at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.