MTHFD2 Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A22653
Artikelname: MTHFD2 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A22653
Hersteller Artikelnummer: A22653
Alternativnummer: ABB-A22653-20UL,ABB-A22653-100UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: NMDMC, MTHFD2
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.
Klonalität: Monoclonal
Molekulargewicht: 38kDa
NCBI: 10797
UniProt: P13995
Reinheit: Affinity purification
Target-Kategorie: MTHFD2
Application Verdünnung: WB,1:2000 - 1:8000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse. ResearchArea: Signal Transduction ,Metabolism ,Mitochondrial Metabolism ,Mitochondrial markers. Shipping: Ice Bag
Western blot analysis of various lysates, using MTHFD2 Rabbit mAb (A22653) at 1:7000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.