MTHFD2 Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A22653
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| Article Name: | MTHFD2 Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A22653 |
| Supplier Catalog Number: | A22653 |
| Alternative Catalog Number: | ABB-A22653-20UL,ABB-A22653-100UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | NMDMC, MTHFD2 |
| This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. |
| Application Dilute: | WB,1:2000 - 1:8000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse. ResearchArea: Signal Transduction ,Metabolism ,Mitochondrial Metabolism ,Mitochondrial markers. Shipping: Ice Bag |
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Western blot analysis of various lysates, using MTHFD2 Rabbit mAb (A22653) at 1:7000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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