MDM2 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:
ABB-A23388
- Bilder (1)
| Artikelname: | MDM2 Rabbit mAb, Unconjugated, Monoclonal |
| Artikelnummer: | ABB-A23388 |
| Hersteller Artikelnummer: | A23388 |
| Alternativnummer: | ABB-A23388-100UL,ABB-A23388-20UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | HDMX, LSKB, hdm2, ACTFS, MDM2 |
| This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. |
| Application Verdünnung: | WB,1:2000 - 1:10000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Cancer,Tumor suppressors,p53 pathway,Signal Transduction,PI3K-Akt Signaling Pathway,ErbB-HER Signaling Pathway,ATM Signaling Pathway,Cell Biology Developmental Biology,Cell Cycle,Cell cycle inhibitors,Cell Cycle Control-G2 M DNA Damage Checkpoint,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,Immunology Inflammation,Neuroscience. Shipping: Ice Bag |
|
|
Western blot analysis of lysates from U-2 OS cells using MDM2 Rabbit mAb (A23388) at 1:5000 dilutionincubated at room temperature for 1.5 hours. U-2 OS cells were treated with Nutlin 3a (10 µM) at 37°C for 24 hrs Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s. |
Produktgarantie und fachkundiger Support
