MDM2 Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A23388
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| Article Name: | MDM2 Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A23388 |
| Supplier Catalog Number: | A23388 |
| Alternative Catalog Number: | ABB-A23388-100UL,ABB-A23388-20UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | HDMX, LSKB, hdm2, ACTFS, MDM2 |
| This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. |
| Application Dilute: | WB,1:2000 - 1:10000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Cancer,Tumor suppressors,p53 pathway,Signal Transduction,PI3K-Akt Signaling Pathway,ErbB-HER Signaling Pathway,ATM Signaling Pathway,Cell Biology Developmental Biology,Cell Cycle,Cell cycle inhibitors,Cell Cycle Control-G2 M DNA Damage Checkpoint,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,Immunology Inflammation,Neuroscience. Shipping: Ice Bag |
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Western blot analysis of lysates from U-2 OS cells using MDM2 Rabbit mAb (A23388) at 1:5000 dilutionincubated at room temperature for 1.5 hours. U-2 OS cells were treated with Nutlin 3a (10 µM) at 37°C for 24 hrs Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s. |
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