pan 14-3-3 Rabbit pAb, Unconjugated, Polyclonal

Artikelnummer: ABB-A23674
Artikelname: pan 14-3-3 Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer: ABB-A23674
Hersteller Artikelnummer: A23674
Alternativnummer: ABB-A23674-100UL,ABB-A23674-500UL,ABB-A23674-20UL,ABB-A23674-1000UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: YWHA, S1C5, HS1, 14-3-3, pan 14-3-3
This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5 UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.
Klonalität: Polyclonal
Molekulargewicht: 27kDa/28kDa
NCBI: 10971
UniProt: P27348
Reinheit: Affinity purification
Target-Kategorie: pan 14-3-3
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat,African green monkey. Shipping: Ice Bag
Western blot analysis of various lysates, using pan 14-3-3 Rabbit pAb (A23674) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.