pan 14-3-3 Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A23674
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| Article Name: | pan 14-3-3 Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A23674 |
| Supplier Catalog Number: | A23674 |
| Alternative Catalog Number: | ABB-A23674-100UL,ABB-A23674-500UL,ABB-A23674-20UL,ABB-A23674-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | YWHA, S1C5, HS1, 14-3-3, pan 14-3-3 |
| This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5 UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. |
| Application Dilute: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat,African green monkey. Shipping: Ice Bag |
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Western blot analysis of various lysates, using pan 14-3-3 Rabbit pAb (A23674) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
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