ATP6V0C Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A23757
Artikelname: ATP6V0C Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A23757
Hersteller Artikelnummer: A23757
Alternativnummer: ABB-A23757-20UL,ABB-A23757-100UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: ATP6V0C, ATP6C, ATP6L, ATPL, VATL, VPPC, Vma3, ATPase H+ transporting V0 subunit c
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c, c, and d. This gene encodes the V0 subunit c. Alternative splicing results in transcript variants. Pseudogenes have been identified on chromosomes 6 and 17.
Klonalität: Monoclonal
Molekulargewicht: 15kDa
NCBI: 527
UniProt: P27449
Reinheit: Affinity purification
Target-Kategorie: ATP6V0C
Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human. ResearchArea: Cancer,Signal Transduction,Endocrine & Metabolism. Shipping: Ice Bag
Western blot analysis of various lysates, using ATP6V0C Rabbit mAb (A23757) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.