[KD Validated] EIF2AK1 Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A24614
Artikelname: [KD Validated] EIF2AK1 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A24614
Hersteller Artikelnummer: A24614
Alternativnummer: ABB-A24614-20UL,ABB-A24614-100UL,ABB-A24614-1000UL,ABB-A24614-500UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: HCR, HRI, hHRI, LEMSPAD, [KD Validated] EIF2AK1
The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.
Klonalität: Monoclonal
Klon-Bezeichnung: [ARC62929]
Molekulargewicht: 71kDa
NCBI: 27102
UniProt: Q9BQI3
Reinheit: Affinity purification
Sequenz: SIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNS
Target-Kategorie: EIF2AK1
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Signal Transduction,Kinase. Shipping: Ice Bag
Western blot analysis of lysates from wild type(WT) and EIF2AK1 knockdown (KD) 293T cells, using [KD Validated] EIF2AK1 Rabbit mAb (A24614) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.