[KD Validated] EIF2AK1 Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A24614
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| Article Name: | [KD Validated] EIF2AK1 Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A24614 |
| Supplier Catalog Number: | A24614 |
| Alternative Catalog Number: | ABB-A24614-20UL,ABB-A24614-100UL,ABB-A24614-1000UL,ABB-A24614-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | HCR, HRI, hHRI, LEMSPAD, [KD Validated] EIF2AK1 |
| The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. |
| Clonality: | Monoclonal |
| Clone Designation: | [ARC62929] |
| Molecular Weight: | 71kDa |
| NCBI: | 27102 |
| UniProt: | Q9BQI3 |
| Purity: | Affinity purification |
| Sequence: | SIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNS |
| Target: | EIF2AK1 |
| Antibody Type: | Primary Antibody |
| Application Dilute: | WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Signal Transduction,Kinase. Shipping: Ice Bag |
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Western blot analysis of lysates from wild type(WT) and EIF2AK1 knockdown (KD) 293T cells, using [KD Validated] EIF2AK1 Rabbit mAb (A24614) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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