MRPL22 Rabbit pAb, Unconjugated, Polyclonal

Artikelnummer: ABB-A24777
Artikelname: MRPL22 Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer: ABB-A24777
Hersteller Artikelnummer: A24777
Alternativnummer: ABB-A24777-20UL,ABB-A24777-100UL,ABB-A24777-1000UL,ABB-A24777-500UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: MRPL22, HSPC158, L22mt, MRP-L22, MRP-L25, RPML25, mitochondrial ribosomal protein L22
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene.
Klonalität: Polyclonal
Molekulargewicht: 13kDa/14kDa/23kDa
NCBI: 29093
UniProt: Q9NWU5
Reinheit: Affinity purification
Sequenz: GEPRRPAEIYHCRRQIKYSKDKMWYLAKLIRGMSIDQALAQLEFNDKKGAKIIKEVLLEAQDMAVRDHNVEFRSNLYIAESTSGRGQCLKRIRYHGRGRFGIMEKVYCHYFVKLVEGPPPPPEPPKTAVAHAKEYIQQLRSRTIVHTL
Target-Kategorie: MRPL22
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:1000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human. ResearchArea: Epigenetics & Nuclear Signaling,Endocrine & Metabolism,Mitochondrial metabolism,Mitochondrial translation. Shipping: Ice Bag
Western blot analysis of lysates from 293T cells usingMRPL22 Rabbit pAb (A24777) at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.