eIF4A1 Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer:
ABB-A5294
- Bilder (8)
| Artikelname: | eIF4A1 Rabbit pAb, Unconjugated, Polyclonal |
| Artikelnummer: | ABB-A5294 |
| Hersteller Artikelnummer: | A5294 |
| Alternativnummer: | ABB-A5294-100UL,ABB-A5294-20UL,ABB-A5294-500UL,ABB-A5294-1000UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IP, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | DDX2A, EIF4A, EIF-4A, eIF4A-I, eIF-4A-I, eIF4A1 |
| Enables double-stranded RNA binding activity. Predicted to be involved in cytoplasmic translational initiation. Located in cytoplasm. |
| Application Verdünnung: | WB,1:1000 - 1:5000|IF/ICC,1:50 - 1:200|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Immunology Inflammation. Shipping: Ice Bag |
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Immunofluorescence analysis of C6 cells using eIF4A1 Rabbit pAb (A5294) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH/3T3 cells using eIF4A1 Rabbit pAb (A5294) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Western blot analysis of various lysates, using eIF4A1 Rabbit pAb (A5294) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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Immunofluorescence analysis of U2OS cells using eIF4A1 Rabbit pAb (A5294) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of C6 cells using eIF4A1 Rabbit pAb (A5294) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH/3T3 cells using eIF4A1 Rabbit pAb (A5294) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of U2OS cells using eIF4A1 Rabbit pAb (A5294) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunoprecipitation analysis of 300 µg extracts of HeLa cells using 3 µg eIF4A1 antibody (A5294). Western blot was performed from the immunoprecipitate using eIF4A1 antibody (A5294) at a dilution of 1:1000. |
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