PIP5K1A Rabbit pAb, Unconjugated, Polyclonal

Artikelnummer: ABB-A7941
Artikelname: PIP5K1A Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer: ABB-A7941
Hersteller Artikelnummer: A7941
Alternativnummer: ABB-A7941-20UL,ABB-A7941-100UL,ABB-A7941-500UL,ABB-A7941-1000UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: PIP5K1A
Enables 1-phosphatidylinositol-4-phosphate 5-kinase activity and kinase binding activity. Involved in several processes, including activation of GTPase activity, focal adhesion assembly, and ruffle assembly. Located in several cellular components, including lamellipodium, nuclear speck, and ruffle membrane. Colocalizes with mRNA cleavage and polyadenylation specificity factor complex.
Klonalität: Polyclonal
Molekulargewicht: 63kDa
NCBI: 8394
UniProt: Q99755
Reinheit: Affinity purification
Sequenz: MNNLLPRSVKMHIKYDLKGSTYKRRASQKEREKPLPTFKDLDFLQDIPDGLFLDADMYNALCKTLQRDCLVLQSFKIMDYSLLMSIHNIDHAQREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDHMGGIPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPGFYAERFQRFMCNTVFKKIPCVHLGRPDVLPQTPPLEEISEGSPIPDPSFSPLVGETLQMLTTS
Target-Kategorie: PIP5K1A
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:2000|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse. ResearchArea: Signal Transduction,Kinase,PI3K-Akt Signaling Pathway,Cell Biology Developmental Biology,Cytoskeleton,Actins,Endocrine Metabolism,Lipid Metabolism. Shipping: Ice Bag
Western blot analysis of lysates from HeLa cells, using PIP5K1A Rabbit pAb (A7941) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.