Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:
Unconjugated
Alternative Names:
PIP5K1A
Enables 1-phosphatidylinositol-4-phosphate 5-kinase activity and kinase binding activity. Involved in several processes, including activation of GTPase activity, focal adhesion assembly, and ruffle assembly. Located in several cellular components, including lamellipodium, nuclear speck, and ruffle membrane. Colocalizes with mRNA cleavage and polyadenylation specificity factor complex.
WB,1:500 - 1:2000|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:
Cross-Reactivity: Human,Mouse. ResearchArea: Signal Transduction,Kinase,PI3K-Akt Signaling Pathway,Cell Biology Developmental Biology,Cytoskeleton,Actins,Endocrine Metabolism,Lipid Metabolism. Shipping: Ice Bag
Western blot analysis of lysates from HeLa cells, using PIP5K1A Rabbit pAb (A7941) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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