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Western blot analysis of various lysates using HSP20/HSPB6 Rabbit mAb (A9091) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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Immunohistochemistry analysis of paraffin-embeddedMouse heart tissue usingHSP20/HSPB6 Rabbit mAb(A9091) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman colon carcinoma tissue usingHSP20/HSPB6 Rabbit mAb(A9091) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman liver cancer tissue usingHSP20/HSPB6 Rabbit mAb(A9091) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedRat colon tissue usingHSP20/HSPB6 Rabbit mAb(A9091) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman lung tissue usingHSP20/HSPB6 Rabbit mAb(A9091) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedRat heart tissue usingHSP20/HSPB6 Rabbit mAb(A9091) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining. |
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Confocal imaging ofparaffin-embedded Rat heart tissue usingHSP20/HSPB6 Rabbit mAb (A9091, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining. |
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Confocal imaging ofparaffin-embedded Mouse heart tissue usingHSP20/HSPB6 Rabbit mAb (A9091, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining. |