HSP20/HSPB6 Rabbit mAb, Unconjugated, Monoclonal

Catalog Number: ABB-A9091
Article Name: HSP20/HSPB6 Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number: ABB-A9091
Supplier Catalog Number: A9091
Alternative Catalog Number: ABB-A9091-100UL,ABB-A9091-20UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, IF, IHC-P, WB
Species Reactivity: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: HEL55, Hsp20, PPP1R91, HSP20/HSPB6
This locus encodes a heat shock protein. The encoded protein likely plays a role in smooth muscle relaxation.
Clonality: Monoclonal
Molecular Weight: 17kDa
NCBI: 126393
UniProt: O14558
Purity: Affinity purification
Target: HSPB6
Application Dilute: WB,1:500 - 1:1000|IF-P,1:50 - 1:200|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Apoptosis,Signal Transduction ,Protein Trafficking ,Chaperones ,Heat Shock Proteins,Cardiovascular, Atherosclerosis ,Ischemia / Reperfusion,Cardiovascular ,Heart Apoptosis. Shipping: Ice Bag
Western blot analysis of various lysates using HSP20/HSPB6 Rabbit mAb (A9091) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embeddedMouse heart tissue usingHSP20/HSPB6 Rabbit mAb(A9091) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embeddedHuman colon carcinoma tissue usingHSP20/HSPB6 Rabbit mAb(A9091) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embeddedHuman liver cancer tissue usingHSP20/HSPB6 Rabbit mAb(A9091) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embeddedRat colon tissue usingHSP20/HSPB6 Rabbit mAb(A9091) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embeddedHuman lung tissue usingHSP20/HSPB6 Rabbit mAb(A9091) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embeddedRat heart tissue usingHSP20/HSPB6 Rabbit mAb(A9091) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
Confocal imaging ofparaffin-embedded Rat heart tissue usingHSP20/HSPB6 Rabbit mAb (A9091, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Confocal imaging ofparaffin-embedded Mouse heart tissue usingHSP20/HSPB6 Rabbit mAb (A9091, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.