DPP6 Antibody - middle region

Artikelnummer: ASB-ARP44867_P050
Artikelname: DPP6 Antibody - middle region
Artikelnummer: ASB-ARP44867_P050
Hersteller Artikelnummer: ARP44867_P050
Alternativnummer: ASB-ARP44867_P050-25UL,ASB-ARP44867_P050-100UL
Hersteller: Aviva
Wirt: Rabbit
Kategorie: Proteine/Peptide
Applikation: WB
Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DPP6
Alternative Synonym: VF2, DPL1, DPPX, MRD33
DPP6 is a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties.This gene encodes a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Klonalität: Polyclonal
Konzentration: 0.5 mg/ml
Molekulargewicht: 98 kDa
NCBI: 1804
UniProt: P42658
Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
WB Suggested Anti-DPP6 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human brain
Lanes:
Lane 1: 20ug HEK-293 cell lysate
Lane 2: 20ug hDPP6-YFP transfected HEK-293 lysate
Lane 3: 20ug hDPP10-YFP transfected HEK-293 lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Donkey anti-rabbit-HRP
Secondary Antibody Dilution:
1:10,000
Gene Name:
DPP6 a
Submitted by:
Jeanne M. Nerbonne, Arvin Soepriatna, Washington University Medical School Department of Developmental Biology
Researcher: Jeanne M. Nerbonne, Arvin Soepriatna, Washington University Medical School
Application: Western blotting
Species+tissue/cell type: Mouse WT and DPP6 -/- brain
How many ugsof tissue/cell lysate run on the gel:
1. 20ug Mouse WT brain extract
2. 20ug Mouse DPP6 -/- brain extract
3: 20ug Mouse WT brain extract
4: 20ug Mouse DPP6 -/- brain extract
Primary antibody dilution: 1:1000
Secondary antibody: Donkey anti-rabbbit HRP
Secondary antibody dilution: 1: 10,000
DPP6 antibody - middle region (ARP44867_P050) in Human Brain using Western Blot