Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
Immunogen:
The immunogen is a synthetic peptide directed towards the middle region of human DPP6
Alternative Names:
VF2, DPL1, DPPX, MRD33
DPP6 is a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties.This gene encodes a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Lanes: Lane 1: 20ug HEK-293 cell lysate Lane 2: 20ug hDPP6-YFP transfected HEK-293 lysate Lane 3: 20ug hDPP10-YFP transfected HEK-293 lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Donkey anti-rabbit-HRP Secondary Antibody Dilution: 1:10,000 Gene Name: DPP6 a Submitted by: Jeanne M. Nerbonne, Arvin Soepriatna, Washington University Medical School Department of Developmental Biology
Researcher: Jeanne M. Nerbonne, Arvin Soepriatna, Washington University Medical School Application: Western blotting Species+tissue/cell type: Mouse WT and DPP6 -/- brain How many ugsof tissue/cell lysate run on the gel: 1. 20ug Mouse WT brain extract 2. 20ug Mouse DPP6 -/- brain extract 3: 20ug Mouse WT brain extract 4: 20ug Mouse DPP6 -/- brain extract Primary antibody dilution: 1:1000 Secondary antibody: Donkey anti-rabbbit HRP Secondary antibody dilution: 1: 10,000
DPP6 antibody - middle region (ARP44867_P050) in Human Brain using Western Blot
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