CD151 Antibody - C-terminal region

Artikelnummer: ASB-ARP63500_P050
Artikelname: CD151 Antibody - C-terminal region
Artikelnummer: ASB-ARP63500_P050
Hersteller Artikelnummer: ARP63500_P050
Alternativnummer: ASB-ARP63500_P050-25UL,ASB-ARP63500_P050-100UL
Hersteller: Aviva
Wirt: Rabbit
Kategorie: Proteine/Peptide
Applikation: IHC, WB
Spezies Reaktivität: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rat
Alternative Synonym: GP27, MER2, RAPH, SFA1, PETA-3, TSPAN24
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene.
Klonalität: Polyclonal
Konzentration: 0.5 mg/ml
Molekulargewicht: 28kDa
NCBI: 977
UniProt: P48509
Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Application: IHC
Species+tissue/cell type:Human nasal epithelial cells
Primary antibody dilution: 1:1000
Secondary antibody: Goat anti-rabbit Alexa Fluor 546
Secondary antibody dilution:1:1000
WB Suggested Anti-CD151 Antibody
Titration: 1.0 ug/ml
Positive Control: A549 Whole CellCD151 is supported by BioGPS gene expression data to be expressed in A549
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
CD151 antibody - C-terminal region (ARP63500_P050) in Human A549 using Western Blot