CD151 Antibody - C-terminal region

Catalog Number: ASB-ARP63500_P050
Article Name: CD151 Antibody - C-terminal region
Biozol Catalog Number: ASB-ARP63500_P050
Supplier Catalog Number: ARP63500_P050
Alternative Catalog Number: ASB-ARP63500_P050-25UL,ASB-ARP63500_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: IHC, WB
Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rat
Alternative Names: GP27, MER2, RAPH, SFA1, PETA-3, TSPAN24
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene.
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 28kDa
NCBI: 977
UniProt: P48509
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Application: IHC
Species+tissue/cell type:Human nasal epithelial cells
Primary antibody dilution: 1:1000
Secondary antibody: Goat anti-rabbit Alexa Fluor 546
Secondary antibody dilution:1:1000
WB Suggested Anti-CD151 Antibody
Titration: 1.0 ug/ml
Positive Control: A549 Whole CellCD151 is supported by BioGPS gene expression data to be expressed in A549
Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
CD151 antibody - C-terminal region (ARP63500_P050) in Human A549 using Western Blot