PPA1 Monoclonal Antibody, IgG1, Clone: [1H4E1], Unconjugated, Mouse
Artikelnummer:
CSB-MA614884A0M
- Bilder (4)
| Artikelname: | PPA1 Monoclonal Antibody, IgG1, Clone: [1H4E1], Unconjugated, Mouse |
| Artikelnummer: | CSB-MA614884A0M |
| Hersteller Artikelnummer: | CSB-MA614884A0m |
| Alternativnummer: | CSB-MA614884A0M-100UL, CSB-MA614884A0M-50UL |
| Hersteller: | Cusabio |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | ELISA, FC, IF, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | cytosolic inorganic pyrophosphatase antibody, diphosphate phosphohydrolase antibody, inorganic diphosphatase antibody, inorganic pyrophosphatase 1 antibody, Inorganic pyrophosphatase antibody, IOPPP antibody, IPYR_HUMAN antibody, PP antibody, PP1 antibody, PPA1 antibody, PPase antibody, Pyp antibody, pyrophosphatase, inorganic, 1 antibody, Pyrophosphate phospho hydrolase antibody, Pyrophosphate phospho-hydrolase antibody, SID6-8061 antibody |
| Klonalität: | Monoclonal |
| Klon-Bezeichnung: | [1H4E1] |
| Isotyp: | IgG1 |
| UniProt: | Q15181 |
| Puffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Reinheit: | >95%, Protein G purified |
| Formulierung: | Liquid |
| Target-Kategorie: | PPA1 |
| Application Verdünnung: | Recommended dilution: WB: 1:1000-1:5000, IF: 1:50-1:200, FC: 1:50-1:200 |
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Overlay Peak curve showing HepG2 cells stained with CSB-MA614884A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
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Overlay Peak curve showing 293T cells stained with CSB-MA614884A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
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Immunofluorescence staining of Hela cells with CSB-MA614884A0m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). |
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Western Blot Positive WB detected in: PPA1 antibody at 1:1000 Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: 293T whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: U87 whole cell lysate Secondary Goat polyclonal to Mouse IgG at 1/20000 dilution Predicted band size: 33KDa Observed band size: 33 KDa Exposure time: 5min |
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