PPA1 Monoclonal Antibody, IgG1, Clone: [1H4E1], Unconjugated, Mouse
Catalog Number:
CSB-MA614884A0M
- Images (4)
| Article Name: | PPA1 Monoclonal Antibody, IgG1, Clone: [1H4E1], Unconjugated, Mouse |
| Biozol Catalog Number: | CSB-MA614884A0M |
| Supplier Catalog Number: | CSB-MA614884A0m |
| Alternative Catalog Number: | CSB-MA614884A0M-100UL, CSB-MA614884A0M-50UL |
| Manufacturer: | Cusabio |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ELISA, FC, IF, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | cytosolic inorganic pyrophosphatase antibody, diphosphate phosphohydrolase antibody, inorganic diphosphatase antibody, inorganic pyrophosphatase 1 antibody, Inorganic pyrophosphatase antibody, IOPPP antibody, IPYR_HUMAN antibody, PP antibody, PP1 antibody, PPA1 antibody, PPase antibody, Pyp antibody, pyrophosphatase, inorganic, 1 antibody, Pyrophosphate phospho hydrolase antibody, Pyrophosphate phospho-hydrolase antibody, SID6-8061 antibody |
| Clonality: | Monoclonal |
| Clone Designation: | [1H4E1] |
| Isotype: | IgG1 |
| UniProt: | Q15181 |
| Buffer: | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purity: | >95%, Protein G purified |
| Form: | Liquid |
| Target: | PPA1 |
| Application Dilute: | Recommended dilution: WB: 1:1000-1:5000, IF: 1:50-1:200, FC: 1:50-1:200 |
|
|
Overlay Peak curve showing HepG2 cells stained with CSB-MA614884A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
|
|
Overlay Peak curve showing 293T cells stained with CSB-MA614884A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
|
|
Immunofluorescence staining of Hela cells with CSB-MA614884A0m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). |
|
|
Western Blot Positive WB detected in: PPA1 antibody at 1:1000 Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: 293T whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: U87 whole cell lysate Secondary Goat polyclonal to Mouse IgG at 1/20000 dilution Predicted band size: 33KDa Observed band size: 33 KDa Exposure time: 5min |
Product Guarantee and Expert Support
