Lyophilized from 0.22 µm filtered solution in 20 mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System:
HEK293
Reinheit:
> 90% as determined by Bis-Tris PAGE,> 85% as determined by HPLC
Formulierung:
Lyophilized
Sequenz:
Leu72-Leu251
Target-Kategorie:
TNFSF15
Human TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Immobilized Human TNFSF15, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.8 ng/ml determined by ELISA.
Human DR3, hFc Tag captured on CM5 Chip via Protein A can bind Human TNFSF15, His Tag with an affinity constant of 4.09 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNFSF15, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA.
The purity of Human TNFSF15 is greater than 85% and the molecular weight of this protein is around 60-90 kDa as determined by SEC-MALS.
Immobilized Human TNFSF15, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 24.2ng/ml determined by ELISA.
Loaded Human DR3, hFc Tag on ProA-Biosensor, can bind Human TNFSF15, His Tag with an affinity constant of 5.04 nM as determined in BLI assay (Gator).
The purity of Human TNFSF15 is greater than 85% as determined by SEC-HPLC.
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