Human TNFSF15 Protein (active trimer) (FSF-HM115)

Catalog Number: KAB-FSF-HM115
Article Name: Human TNFSF15 Protein (active trimer) (FSF-HM115)
Biozol Catalog Number: KAB-FSF-HM115
Supplier Catalog Number: FSF-HM115
Alternative Catalog Number: KAB-FSF-HM115-100UG, KAB-FSF-HM115-500UG
Manufacturer: KactusBio
Category: Proteine/Peptide
Species Reactivity: Human
Alternative Names: TL1A, VEGI-251, TNFSF15, TL1, VEGI, VEGI192A
Recombinant Human TNFSF15 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu72-Leu251.
Molecular Weight: The protein has a predicted MW of 21.57 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
Tag: N-His
UniProt: O95150
Buffer: Lyophilized from 0.22 µm filtered solution in 20 mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System: HEK293
Purity: > 90% as determined by Bis-Tris PAGE,> 85% as determined by HPLC
Form: Lyophilized
Sequence: Leu72-Leu251
Target: TNFSF15
Human TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Immobilized Human TNFSF15, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.8 ng/ml determined by ELISA.
Human DR3, hFc Tag captured on CM5 Chip via Protein A can bind Human TNFSF15, His Tag with an affinity constant of 4.09 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNFSF15, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA.
The purity of Human TNFSF15 is greater than 85% and the molecular weight of this protein is around 60-90 kDa as determined by SEC-MALS.
Immobilized Human TNFSF15, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 24.2ng/ml determined by ELISA.
Loaded Human DR3, hFc Tag on ProA-Biosensor, can bind Human TNFSF15, His Tag with an affinity constant of 5.04 nM as determined in BLI assay (Gator).
The purity of Human TNFSF15 is greater than 85% as determined by SEC-HPLC.