Biotinylated Human IL-17A/CTLA-8 Protein (ILA-HM418B)

Artikelnummer: KAB-ILA-HM418B
Artikelname: Biotinylated Human IL-17A/CTLA-8 Protein (ILA-HM418B)
Artikelnummer: KAB-ILA-HM418B
Hersteller Artikelnummer: ILA-HM418B
Alternativnummer: KAB-ILA-HM418B-100UG, KAB-ILA-HM418B-500UG
Hersteller: KactusBio
Kategorie: Proteine/Peptide
Spezies Reaktivität: Human
Konjugation: Biotin
Alternative Synonym: CTLA-8, IL17, IL17A, IL-17CTLA-8, interleukin 17A
Recombinant Biotinylated Human IL-17A/CTLA-8 Protein is expressed from HEK293 with Avi tag and His tag at the C-Terminus.It contains Gly24-Ala155.
Molekulargewicht: The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 16-30 kDa based on Bis-Tris PAGE result.
Tag: C-Avi-His
UniProt: Q16552
Puffer: Lyophilized from 0.22µm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Expression System: HEK293
Reinheit: > 95% as determined by Bis-Tris PAGE,> 95% as determined by HPLC
Formulierung: Lyophilized
Sequenz: Gly24-Ala155
Target-Kategorie: IL-17A/CTLA-8
Biotinylated Human IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Human IL-17R alpha, hFc Tag captured on CM5 Chip via protein A can bind Biotinylated Human IL-17A, His-Avi Tag with an affinity constant of 2.18 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human IL-17A, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human IL-17A, His Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
The purity of Biotinylated Human IL-17A is greater than 95% as determined by SEC-HPLC.